D.  
Any time the fuel tanks are entered, inspect the tanks for microbial growth.

 E.  
The Corrosion Prevention Manual, (CPM 20-62-00, Part I) contains a procedure on standard preventive maintenance methods for microbial growth in fuel tanks.

 TASK 28-11-00-207-032
 2.  Detection Check for Contamination With Microbes or Fungus
_________________________________________________________
 A.  General
 (1)  
This procedure uses a Microb-Monitor Test Kit to do a check of the fuel for contamination. The kit contains two bottles. One bottle contains liquid to keep the microbes. The other bottle contains an equivalent liquid with a biocide added to it.

 (2)  
Make sure to sample each fuel tank.

 B.  
Special Tools and Equipment

 (1)  
Use one of these microbial growth detection test kits:

 NOTE: The test kits are equivalent.
____
 (a)  Microb-Monitor Test Kit,
 Boron Oil Co. Div. of BP Co. Air BP Cleveland Hopkins Intl. Airport Cleveland, Ohio, USA. 44135
 Telephone: 216-267-3550
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BOEING PROPRIETARY - Copyright (C) - Unpublished Work - See title page for details.
 C.
D.
E.
F.
A
747-400 MAINTENANCE MANUAL
 (b) Hum-Bug Detector Kit
 SIL-MID, Limited (Supplier) Unit 29 Elmdon Trading Estate Bickenhill Lane Birmingham, England B377HE
 Telephone: 021-781-0202 FAX: 021-781-0281
 Hum-Bug Detector Kit
 Hammonds Fuel Additives (Manufacturer)
 P. O. Box 38114-373 Houston, Texas, USA 77238-8114
 Telephone: 713-442-3777 FAX: 713-442-3974
 (c) Easicult Combi (Detector Kit)
 Orion Diagnostica (Manufacturer)
 P.O. Box 83 02101 Espoo, Finland
 Telephone: 358-9-429-2888 FAX: 358-9-429-2794
Standard Tools and Equipment
 (1)
Syringe - hypodermicReferences

 (1)
AMM 12-11-03/301, Fuel Tank Sumping

 (2)
AMM 28-11-00/201, Fuel TankConsumable Materials

 (1)
B00130 Alcohol - Isopropyl, to clean the equipmentAccess

 (1)  
Location Zones 131 Center Section Wing Box, Left 132 Center Section Wing Box, Right 500 Left Wing 600 Right Wing

 (2)  
Access Panels 191G Fuel Drain and Dripstick Access Door 192G Fuel Drain and Dripstick Access Door

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BOEING PROPRIETARY - Copyright (C) - Unpublished Work - See title page for details.
A
747-400 MAINTENANCE MANUAL
 G. Procedure
 S 757-087
 (1)  
Get a fuel sample for the contamination detection test:

 (a)  
Sterilize the fuel sampling equipment and glass bottles for the fuel sampling test with isopropyl alcohol. Dry completely.

 (b)  
Use the fuel sump drains to get a fuel sample for each fuel tank (AMM 12-11-03/301).

 (c)  
Make sure the fuel sample contains some water and some fuel.

 NOTE: Microbial growth in the fuel tanks occurs at the
____ water-fuel interface.
 S 287-027
 (2)  
Use the Microb-Monitor Test Kit

 (a)  
Put a fuel sample in the two bottles with a hypodermic syringe.

 S 217-028
 (3) After 48 hours, examine the fuel samples for a change in color.
 NOTE: If the fuel samples change colors, the fuel sample has
____
 some contamination.

 TASK 28-11-00-207-177
 3. Fuel Tank Check for Contamination With Microbes or Fungus
_________________________________________________________
 A. General
 (1)  
If the fuel sample for a fuel tank is positive (potential microbial growth), then you must go into the tanks to verify the presence of microbial growth. If you find microbial growth, schedule a task to remove the contamination as soon as possible. If you wait too long to remove the contamination, you expose the tank structure and components to a higher probability of corrosion.
　
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